The aim of this test is to reject all spots with a too low intensity. This Doelan test use the 635 foreground intensity (Genepix F635 Median column) and the 532 foreground intensity (Genepix F532 Median column).
Minimal spot intensity: The mininum value of the intensity in each color for a spot to be not excluded.
Channel to test: The channel(s) to test (red, green or both).
Maximal threshold of bad spots: The threshold in percent of invalid spots to reject the test. In the count, empty and abscent spots aren't considered.
Remove bad spot from the output list: If enabled, spots which don't pass the test will be removed in output arraylist file.
This test will generate two graphics (one for each color) showing the distribution of intensities of the spots.