Basic Statistics
Measure | Value |
---|---|
Filename | cond1rep1_S1_L003_R2_001.fastq.bz2 |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1805703 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGACAGACTGCAGCCATTCGCTCACTGATGAGGAGTAGCAGTGTTGGACGGCTCCCAAGGCGGATGTGGTACAGT | 3548 | 0.1964885698257133 | Search with Blastn,more detail First hit on 16: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
CACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAA | 3131 | 0.1733950710609663 | Search with Blastn,more detail First hit on 14: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
CCAGTAAAGACTTAGTGGGGAACTGCAAAAGCCACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAG | 2950 | 0.16337127423502093 | Search with Blastn,more detail First hit on 44: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
GGGGAACTGCAAAAGCCACATGACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGA | 2811 | 0.15567344131343858 | Search with Blastn,more detail First hit on 45: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG | 2711 | 0.1501354320173362 | Illumina RNA PCR Primer (100% over 50bp) |
ACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAAAAAATA | 2522 | 0.13966859444770266 | Search with Blastn,more detail First hit on 14: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
GGAGAAATATACAGAATGTAGAAAAGTCCTAACAGTCCACTGTGCCCGGGGGTGTAGCCACTTCCTTTCCTTCCC | 2518 | 0.13944707407585855 | Search with Blastn,more detail First hit on 16: Mus musculus targeted non-conditional, lacZ-tagged mutant allele Pcp4l1:tm1e(KOMP)Wtsi; transgenic Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
ACGTCATTGGTCCTCGGTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAGATCGTC | 2496 | 0.13822871203071602 | No hit |
CGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCG | 2349 | 0.1300878383654455 | Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
GGAGTCTTAGCATCAGAAGGACACCGTATATACATCTACAGTTGGTGGCCAATACAAGTCATCGCCAGACAGTCC | 2084 | 0.1154121137307741 | Search with Blastn,more detail First hit on 76: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
GTCATGGGGCCGTGGGCTCAGGTAAACAAACGAAAGAATGGATGGGCAAATAGATCGTCGGACTGTAGAACTCTG | 1937 | 0.10727124006550358 | Illumina DpnII expression Sequencing Primer (100% over 23bp) |
GGGGTTTGTCCCCACACTTTGGTTATCCTATGAGGTTGAGTGGGAGGGAGGTATCTGCTTTCAGATGAAAGGGGG | 1861 | 0.10306235300046573 | Search with Blastn,more detail First hit on 70: Mus musculus peripheral myelin protein 22 (Pmp22), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAAA | 6250 | 0.0 | 12.779562 | 47 |
AAACG | 6725 | 0.0 | 12.1405115 | 44 |
AACGA | 6710 | 0.0 | 12.008943 | 45 |
CGCGA | 1435 | 0.0 | 11.872842 | 57 |
ACGAA | 7895 | 0.0 | 10.341347 | 46 |
CGGTC | 8545 | 0.0 | 9.929407 | 15 |
CGTCA | 8565 | 0.0 | 9.790553 | 2 |
GGCCG | 8450 | 0.0 | 9.534774 | 24 |
TACCG | 2480 | 0.0 | 9.44778 | 14 |
CGTGG | 9035 | 0.0 | 9.270969 | 27 |
CCGTG | 9360 | 0.0 | 9.138659 | 26 |
CCTCG | 9315 | 0.0 | 8.954189 | 12 |
GCCGT | 9290 | 0.0 | 8.901875 | 25 |
ACGTC | 7580 | 0.0 | 8.731822 | 1 |
CTAAC | 6535 | 0.0 | 8.635606 | 29 |
TTCGC | 3090 | 0.0 | 8.616925 | 17 |
CCGCG | 2125 | 0.0 | 8.017438 | 56 |
GACGT | 5140 | 0.0 | 8.010955 | 6 |
GGAGA | 18385 | 0.0 | 7.993694 | 1 |
CGGGG | 7410 | 0.0 | 7.904587 | 47 |
Bad tiles
No bad tiles