cond2rep1_S4_L001_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep1_S4_L001_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1960334
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	5502	0.2806664578587118	Illumina RNA PCR Primer (100% over 50bp)
AGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAA	2931	0.14951533769245445	Search with Blastn,more detail First hit on +100: Rhinebothroides cf. freitasi LRP3929 28S ribosomal RNA gene, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
GGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC	2839	0.14482225988020409	Search with Blastn,more detail First hit on +100: TPA_exp: Mus musculus ribosomal DNA, complete repeating unit Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA	2496	0.12732524151496633	Search with Blastn,more detail First hit on +100: Homo sapiens clone LA13_165F6 sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3075	12.813746	47
CCGTA	4045	10.179764	46
GTCGC	5155	8.056667	42
ATACG	3110	7.87566	36
ATCGA	4455	7.489941	60
TACCG	2725	7.4251733	6
GGAAA	19540	7.344744	1
TAGCG	3675	7.0512114	26
CGCAA	3500	6.7952433	32
TACGA	3795	6.734711	37
CAACG	4755	6.7187767	16
AACGC	4610	6.6221004	17
GGAGA	19280	6.335535	1
GTATC	7440	6.1548157	48
CGCCG	6840	6.123844	44
CGAAT	4120	6.1172943	39
CGACA	5395	6.0533333	56
GGTCG	8685	5.803858	41
CGCTT	5750	5.7413416	19
ACGCT	5650	5.717303	18

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