FastQCFastQC Report
Wed 21 Sep 2016
cond2rep2_S5_L002_R2_001.fastq.bz2

Summary

[OK]Basic Statistics

MeasureValue
Filenamecond2rep2_S5_L002_R2_001.fastq.bz2
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829940
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG35730.19525230335420835Illumina RNA PCR Primer (100% over 50bp)
AATGACGCTCGAACAGGCATGCCCCATGGAATACCAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACTG30430.16628960512366525Search with Blastn,more detail
First hit on +100: Phoma sp. BPS96 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAT30550.010.45744847
GTGCG35000.09.93922348
CGCAA31750.09.50319542
CCGTA36350.08.98416846
ATTCG36500.08.3637360
GACGC38050.08.3053964
CGAAC38950.07.83764210
GCGCA48950.07.75935341
GTCGC43950.07.753660742
TGCGT44450.07.666442449
GCGTT43400.07.36117650
GGAAA179300.07.30490451
TGACG46950.07.1126423
CGCCG48950.07.10669744
ACGCT51050.07.09249165
GGCGC47850.06.67659440
TTCGA50300.06.56310661
TCGAA52100.06.06382569
TCGCC60250.06.009489543
GGAAG246750.05.97159241

[OK]Bad tiles

No bad tiles

Produced by FastQC (version 0.11.5)