cond2rep2_S5_L004_R1_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep2_S5_L004_R1_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847516
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	5266	0.28503136102745524	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	3173	0.17174411480062962	TruSeq Adapter, Index 5 (100% over 63bp)
TGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTC	2465	0.13342238984669144	Search with Blastn,more detail First hit on +100: PREDICTED: Cavia porcellus collagen alpha-1(I) chain-like (LOC100717127), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCC	5990	8.652728	22
CGTAT	3280	7.251306	45
CTAGG	7275	7.1731744	50
GTCCG	4165	7.074408	18
CGAAC	4145	6.8516073	50
GGTCG	4190	6.693115	14
TATAT	10945	6.6511145	1
TCGTA	3510	6.573876	44
GTCGT	4845	6.5209594	15
GCGAA	4440	6.476156	37
TCGGT	4655	6.4058223	12
AATCG	4615	6.3075023	10
TCGAA	4790	6.299563	49
CGGTC	4760	6.1899395	13
CCTAG	8775	6.1897244	49
GACGT	8230	6.168268	20
GGCGA	5495	5.8788037	36
TATTA	9685	5.7555366	3
CCCTA	9940	5.678554	48
TCGTC	5890	5.6653643	16

No bad tiles