Basic Statistics
Measure | Value |
---|---|
Filename | cond2rep3_S6_L002_R2_001.fastq.bz2 |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1929172 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG | 12180 | 0.6313589457031307 | Illumina RNA PCR Primer (100% over 50bp) |
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA | 3268 | 0.1693990997173917 | Search with Blastn,more detail First hit on +100: Homo sapiens clone LA13_165F6 sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
CGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCG | 2068 | 0.1071962479239798 | Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0% |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTAT | 4010 | 0.0 | 22.484575 | 47 |
CCGTA | 4285 | 0.0 | 21.290098 | 46 |
GTCGC | 5485 | 0.0 | 16.632284 | 42 |
CGCCG | 7315 | 0.0 | 12.277263 | 44 |
CGGTG | 7755 | 0.0 | 12.175735 | 37 |
TCGCC | 8010 | 0.0 | 12.098332 | 43 |
GTATC | 7660 | 0.0 | 12.095035 | 48 |
GCCGT | 8235 | 0.0 | 11.164301 | 45 |
TCGGT | 8540 | 0.0 | 11.056538 | 36 |
GGTCG | 9020 | 0.0 | 10.861703 | 41 |
AACGT | 8875 | 0.0 | 10.479204 | 24 |
CGTGT | 9275 | 0.0 | 10.027271 | 26 |
GAACG | 11105 | 0.0 | 9.557581 | 23 |
ACGTG | 9915 | 0.0 | 9.415826 | 25 |
ATACG | 3325 | 0.0 | 8.967739 | 36 |
TAGCG | 3465 | 0.0 | 8.912743 | 26 |
TATCA | 11145 | 0.0 | 8.790718 | 49 |
TACGA | 3545 | 0.0 | 8.511343 | 37 |
CATTA | 11665 | 0.0 | 8.337987 | 52 |
TCTCG | 11935 | 0.0 | 8.119618 | 34 |
Bad tiles
No bad tiles