Quality Test report

Chip type: Yeast
Test suite: Self-hybridization

Report date: 01 July 2005 11:29:49
Scanned on: 21 February 2005 11:47:58
Scanned by: GenePix 4000 (Emulated)
Array name: unknown
Array revision: unknown
Manufacturer: unknown
Doelan version: 1.0
Doelan compilation date: 01 July 2005 09:36:45

Summary

Test name	Description	635	532	Threshold	Result 635	Result 532	Global Result
Saturated pixels	Test saturated pixels of spots	0.00 %	0.00 %	<= 10.00 %	Pass	Pass	Pass
Heterogeneity	Test Heterogeneity of features	26.85 %	28.79 %	<= 10.00 %	Fail	Fail	Fail
Abscent flag	Test for absent feature flag (Flag=-75)	0.00 %		<= 5.00 %			Pass
Minimal diameter	Test minimal diameter of features	18.94 %		<= 10.00 %			Fail
Maximal diameter	Test maximal diameter of features	0.29 %		<= 10.00 %			Pass
Not found feature	Test for not found feature flag (Flag=-50)	1.02 %		<= 7.00 %			Pass
Minimal intensity	Test if the spots have the required minimal intensity	0.06 %		<= 10.00 %			Pass
Bad spots	Count the maximal bad spots in a test suite	47.89 %		<= 10.00 %			Fail
Bad spots removed	Count the maximal bad spots removed in a test suite	47.89 %		<= 10.00 %			Fail

Test suite result: Fail

New output Array list: Yes
New Array list generator message: The output array contains 8064 features of the 8064 features of the original file.
Empty features: 3862
Real features: 4202
Total features: 8064

Tests

Test: Saturated pixels

Description: Test saturated pixels of spots
Test module: SaturatedPixelsTest
Parameters:
- Maximal threshold of bad spots: 10.0 %
- Threshold maximum saturated pixels: 1 %
- Remove bad spots from output array list: Yes
Result 635 channel : Pass
Result 532 channel: Pass
Comment:
Features with invalid saturated pixels in 532 : 0/8064 features (threshold: 806 features)
Features with invalid saturated pixels in 635: 0/8064 features (threshold: 806 features)

Test: Heterogeneity

Description: Test Heterogeneity of features
Test module: HeterogeneousFeatureTest
Parameters:
- Minimal value of standard deviation: 500
- Maximal threshold of bad spots: 10.0 %
- Remove bad spots from output array list: Yes
Result graph:
Result 635 channel : Fail
Result 532 channel: Fail
Comment:
Heterogeneous features in 532: 2322/8064 features (threshold: 806 features)
Heterogeneous features in 635 : 2165/8064 features (threshold: 806 features)

Test: Abscent flag

Description: Test for absent feature flag (Flag=-75)
Test module: AbsentSpotTest
Parameters:
- Maximal threshold of bad spots: 5.0 %
- Remove bad spots from output array list: Yes
Result: Pass
Comment:
Abscent features: 0/8064 features (threshold: 403 features)

Test: Minimal diameter

Description: Test minimal diameter of features
Test module: MinDiameterFeatureTest
Parameters:
- Minimal diameter value: 80 µm
- Maximal threshold of bad spots: 10.0 %
- Remove bad spots from output array list: Yes
Result graph:
Result: Fail
Comment:
Bad diameter features: 1527/8064 features (threshold: 806 features)

Test: Maximal diameter

Description: Test maximal diameter of features
Test module: MaxDiameterFeatureTest
Parameters:
- Maximal diameter value: 140 µm
- Maximal threshold of bad spots: 10.0 %
- Remove bad spots from output array list: Yes
Result graph:
Result: Pass
Comment:
Bad diameter features: 23/8064 features (threshold: 806 features)

Test: Not found feature

Description: Test for not found feature flag (Flag=-50)
Test module: NotFoundTestFeatureTest
Parameters:
- Maximal threshold of bad spots: 7.0 %
- Remove bad spots from output array list: Yes
Result: Pass
Comment:
Not found features: 82/8064 features (threshold: 564 features)

Test: Minimal intensity

Description: Test if the spots have the required minimal intensity
Test module: ValidSpotingTest
Parameters:
- Minimal spot intensity: 50
- Maximal threshold of bad spots: 10.0 %
- Remove bad spots from output array list: Yes
Result graph:
Result: Pass
Comment:
Features with invalid invalid intensity : 5/8064 features (threshold: 806 features)

Test: Bad spots

Description: Count the maximal bad spots in a test suite
Test module: GlobalMaxBadFeaturesTest
Parameters:
- Maximal threshold of bad spots: 10.0 %
Result: Fail
Comment:
Bad diameter features: 3862/8064 features (threshold: 806 features)

Test: Bad spots removed

Description: Count the maximal bad spots removed in a test suite
Test module: GlobalMaxBadFeaturesRemovedTest
Parameters:
- Maximal threshold of bad spots: 10.0 %
Result: Fail
Comment:
Bad diameter features: 3862/8064 features (threshold: 806 features)