Heterogeneity feature test

Description

The aim of this test is to reject all spots which are too Heterogeneous. This Doelan test use the standard deviation of the green foreground (Genepix F635 SD column) and the standard deviation of the red foreground (Genepix F532 SD column).

Parameters

  • Maximal value of standard deviation: The maximal value of the standard deviation for a spot to be not rejected.
  • Maximal threshold of bad spots: The threshold in percent of invalid spots to reject the test. In the count, empty and abscent spots aren't considered.
  • Remove bad spot from the output list: If enabled, spots which don't pass the test will be removed in output arraylist file.

Graphics

This test will generate two graphics (one for each color) showing the distribution of the standard deviation of the intensities of the spots.